Innoprot ischemic stroke in vitro model enables the analysis of test compounds which protect primary neurons against induced hypoxic conditions. Our in vitro phenotypic assay comprises the analysis of the different parameters associated to cellular damage; including caspase activation, membrane damage, cell viability, mitochondrial damage and Nuclear damage.

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro assay is based on the exposure of rat primary neurons under Oxygen-Glucose Deprivation conditions. Ischemia-like conditions are induced by replacing the normal O2/CO2 equilibrated medium with 94,7% N2 /5% CO2/0,3% O2 medium in a hypoxic chamber. We also use a culture medium without glucose to expose the cells to real OGD…