Innoprot offers phenotypic assays to better understand the complex interplay of networks and pathways analyzing different cellular processes. The development of these cell-based assays comprises primary cells, but also recombinant cell lines mimmicking disease pathways.

At Innoprot we have also developed complex in vitro disease models for different diseases; such as Alzheimer’s Disease, Parkinson’s Disease, Amyotrophic Lateral Sclerosis (ALS) and Fibrosis.

Caspase 3-7 Activity Assay

Caspase 3-7 Activity Assay

Apoptosis, or programmed cell death, is a normal component of the development and health of multicellular organisms. Caspase 3-7 Activity Assay from Innoprot quantifies the apoptotic level in living cells. Apoptosis evaluation study is a cellular assay to evaluate the activation level of the caspase 3 protein which is a key role component during apoptosis…

Oxidative Stress Assay

The oxidative stress is an imbalance between the production of reactive oxygen strains (ROS); and the capacity of the cell to remove them. The excess of these strains finally produce peroxides and free radicals that damage cell components and compromise the cell functions. Oxidative Stress Assay from Innoprot uses a specific probe (CM-H2DCFDA) to evaluate…

Nephrotoxicity Assay

Nephrotoxicity Assay

Proximal tubules of the kidneys are one of the most common targets of nephrotoxic drugs and chemicals. Screens to predict nephrotoxic potential of compounds with insights to mechanisms of toxicity facilitate lead optimization, guide structure-activity relationships, minimize risks of clinical nephrotoxicity and therefore are valuable in the process of drug discovery. Innoprot has developed an…

Cardiotoxicity Assay

Cardiotoxicity Assay

Cardiotoxicity is a condition when the heart has problems to pump properly. One of the most common events for a heart dysfunction is the toxicity of the functional cardiac cells –cardiomyocytes-. The most usual mechanisms involved in the cardiac cell dysfunction are; the oxidative extress, the DNA damage and the caspase activation with the subsequent…

Neurotoxicity Assay

Neurotoxicity Assay

Neurotoxicity is the capacity of certain compounds to affect the normal activity of the neurons. The mechanism involving the neuron citotoxicity can be several: oxidative stress, DNA damage, cytoskeleton dysfunction or apoptosis. We analyze the following parameters in the Neurotoxicity Assay: Cell Viability Apoptosis: Caspase 3/7 Activation Membrane Damage Neurite Outgrowth Mitochondrial Damage In this…

Hepatotoxicity Assay

Hepatotoxicity Assay

Drug-Induced Liver Injury (DILI) is the most common event causing during the drug withdrawals. But the causes of the hepatic failure provoked by the chemical compounds could be several. Due to this reason, a multiparametric assay is needed to predict toxicities that could be hidden in a simple one parameter assay. We analyze the following…

Aβ1-40 Induced Neurotoxicity Assay

Aβ1-40 Induced Neurotoxicity Assay

Intoxication of neurons with beta-amyloid (1-40) is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta peptide. Aβ1-40 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective potential…

Aβ1-42 Induced Neurotoxicity Assay

Aβ1-42 Induced Neurotoxicity Assay

Intoxication of neurons with beta-amyloid (1-42) oligomers is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta oligomers. Aβ1-42 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective…

In vitro Cell Migration Assay

In vitro Cell Migration Assay

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix. In vitro cell migration assay from Innoprot is a Boyden Chamber assay. Boyden Chambers consist of a cell culture insert nested in the…