Innoprot offers phenotypic assays to better understand the complex interplay of networks and pathways analyzing different cellular processes. The development of these cell-based assays comprises primary cells, but also recombinant cell lines mimmicking disease pathways.
At Innoprot we have also developed complex in vitro disease models for different diseases; such as Alzheimer’s Disease, Parkinson’s Disease, Amyotrophic Lateral Sclerosis (ALS) and Fibrosis.
Neurite outgrowth is a key process during neurogenesis. During development, neurons extend numerous processes that differentiate into dendrites and axons. These processes, also termed neurites, are critical for communication between neurons. The initiation of neurite protrusion and subsequent elongation involves complex intracellular signaling. Neurites are particularly interesting in relation to neuropathological disorders, neuronal injury/regeneration, and…
Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.
tau phosphorylation assay from Innoprot allows the identification of tau kinase and phosphatase inhibitors and modulators that affect the behaviour or location of tau protein. Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing. They accumulate in neurofibrillary tangles in Alzheimer’s disease (AD) brain. There is significant evidence that…