About 10% of ALS cases are inherited; and a large subset of them are caused by mutations in the gene encoding the copper-zinc superoxide dismutase (SOD1). The detection of SOD1-positive inclusions in familial ALS patients suggests the role of SOD1 aggregation underlying the pathology of familial ALS. Although SOD1 mutant proteins are different in structure,…
α-Synuclein is a presynaptic neuronal protein that is linked genetically and neuropathologically to Parkinson’s disease (PD). Aggregated a-synuclein is the major component of inclusions in Parkinson’s disease; but also in other synucleinopathy brains indicating that α-synuclein aggregation is associated with the pathogenesis of neurodegenerative disorders. In this assay from Innoprot, we use a SH-SY5Y cell…
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of the pandemic coronavirus disease 2019 (COVID-19). As the first step of the viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor. So, the blockage of…
Our Astrocytosis assay enables the study of fibrosis-related parameters on TGF-β1 induced Human primary astrocytes. Astrocytosis assay from Innoprot allows the evaluation of therapeutic compounds in this disease area by measuring several biomarkers; including Fibronectin, ASMA and also Collagen via high content analysis. Astrocytosis is a common neurocellular manifestation of brain pathology in individuals with…
Cardiac Fibrosis Assay from Innoprot enables monitoring TGF-β/Smad signaling activation on Human Cardiac Fibroblasts. Immortalized primary Human Cardiac Fibroblasts stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in Smad Binding Elements (SBE) expression; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is…
Renal Fibrosis Assay from Innoprot enables the study of fibrosis-related parameters on TGF-β induced Renal Tubular Epithelial Cells. Human Renal Tubular Epithelial Cells stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in αSMA levels; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger…
Endothelial Cell Tube Formation Assay from Innoprot allows to analyze the effect of test-compounds to induce/inhibit new blood vessels formation. We determine the IC50 values of your compounds of interest in whole cell assays using different organ-specific endothelial cells; such as liver, renal, retinal, colonic, lung, pancreas or umbilical endothelial cells. We offer over 20…
Tumor Cell Proliferation Assay from Innoprot includes allows to analyze the effect of test-compounds to increase/decrease cell proliferation. We determine the IC50 values of your compounds of interest in whole cell assays using different techniques; such as MTT, LDH release, CellTiter or nuclei quantification. We offer over 60 different tumor cell lines for cell screening…
Tumor Cell Adhesion Assay from Innoprot allows to analyze the effect of drugs that could affect the physiological interaction between tumor and stromal cells. Besides this assay is very useful to identify adhesion molecules involving in the process of interaction with stromal cells or extracellular matrix substrates. Therefore, an in vitro cell co-culture system for…