Cholecystokinin CCK2 receptor assay

Cholecystokinin CCK2 receptor assay

CCK2R (CCKBR) Human Cholecystokinin receptor assay from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The cholecystokinin B receptor (CCKBR, CCK2R) is a protein that in humans is encoded by the CCKBR gene. This gene encodes a G-protein-coupled receptor for…

Neurotensin Receptor 1 Assay

Neurotensin Receptor 1 Assay

Human Neurotensin receptor 1 assay (NTSR1) from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay. Neurotensin receptor 1 belongs to…

TDP-43 Stress Granules Assay

TDP-43 Stress Granules Assay

Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative disease of the motor neuron system. The TDP-43 pathology seems to be a dominant type of pathology across sporadic ALS types. Our TDP-43 Stress Granules Assay allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model also permits to monitor…

FUS/TLS Stress Granules Assay

FUS/TLS Stress Granules Assay

FUS/TLS Stress Granules Assay from Innoprot requires a stable cell line expressing fluorescent FUS/TLS. During the assay, we incubate cells with test compounds and Sodium Arsenite to induce the FUS/TLS aggregation. Finally, we quantify fluorescent FUS/TLS nuclear globs using the BD Pathway HCS Reader and Attovision Compartimentalization Software. Amyotrophic lateral sclerosis (ALS) is one of…

Excitotoxicity in vitro assay

Excitotoxicity in vitro assay

A brief exposure to glutamate in vitro causes neuronal death, which makes glutamate a useful tool to evaluate neuroprotective activities. Innoprot excitotoxicity in vitro assay offers the advantage of a rapid analysis of the neuroprotective potential of a molecule. In our assay, we incubate rat primary neurons with test compounds 24 hours before L-glutamate insult.…