In vitro Cell Migration Assay

In vitro Cell Migration Assay

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix. In vitro cell migration assay from Innoprot is a Boyden Chamber assay. Boyden Chambers consist of a cell culture insert nested in the…

In vitro Cell Invasion Assay

In vitro Cell Invasion Assay

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix. In vitro cell invasion assay from Innoprot is a Boyden Chamber assay. Boyden Chambers consist of a cell culture insert nested in the…

alpha1A adrenoreceptor assay

alpha1A adrenoceptor assay

alpha1A Adrenoceptor assay (ADRA1A) from Innoprot provides the analysis of both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The alpha-1A adrenergic receptor (α1A adrenoreceptor), also known as ADRA1A, is member of Alpha-1-adrenergic receptors (alpha-1-ARs) family. They activate mitogenic responses and regulate growth and proliferation of…

alpha1B adrenoreceptor assay

alpha1B adrenoceptor assay

alpha1B Adrenoceptor assay (ADRA1B) from Innoprot provides the analysis of both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The alpha-1B adrenergic receptor (α1B adrenoceptor), also known as ADRA1B, is member of Alpha-1-adrenergic receptors (alpha-1-ARs) family. They activate mitogenic responses and regulate growth and proliferation of…

Cholecystokinin CCK2 receptor assay

Cholecystokinin CCK2 receptor assay

CCK2R (CCKBR) Human Cholecystokinin receptor assay from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The cholecystokinin B receptor (CCKBR, CCK2R) is a protein that in humans is encoded by the CCKBR gene. This gene encodes a G-protein-coupled receptor for…

Neurotensin Receptor 1 Assay

Neurotensin Receptor 1 Assay

Human Neurotensin receptor 1 assay (NTSR1) from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay. Neurotensin receptor 1 belongs to…

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro assay is based on the exposure of rat primary neurons under Oxygen-Glucose Deprivation conditions. Ischemia-like conditions are induced by replacing the normal O2/CO2 equilibrated medium with 94,7% N2 /5% CO2/0,3% O2 medium in a hypoxic chamber. We also use a culture medium without glucose to expose the cells to real OGD…

Amyloid-beta peptide Oligomerization Assay

Amyloid-beta peptide Oligomerization Assay

Amyloid-beta peptide Oligomerization Assay is a functional cell-based assay to identify inhibitors of amyloid peptide oligomerization process. We incubate rat primary neurons, human Microglia or SH-SY5Y cells with test compounds in parallel with Aβ1-42 peptide during 24h. After that, we measure Aβ1-42 oligomerization process analysing the number of thiazine staining oligomers related to cell number .

APP Processing Assay

APP Processing Assay

Alzheimer’s Disease (AD) comprises the accumulation of brain depositions of the beta amyloid (βA). The βA is the amyloid precursor protein (APP) digestion product, released from the cell after secretases proteolysis.This is the basis of the APP Processing Assay offered by Innoprot. Innoprot has developed a novel fluorescence-based assay for screening of inhibitors of the…