5-HT2A Serotonin Receptor Assay (HTR2A) from Innoprot allows to assay compounds, or analyze their capability to modulate HTR2A. When a ligand binds to the HTR2A, it activates a G protein; which in turn, triggers a cellular response mediated by calcium. The binding of an agonist to HTR2A induces mobilization of intracellular calcium and also activation of PKC via the PLC–IP3 and DAG pathways, respectively.
5-HT2A Serotonin Receptor Assay comprises the measurement of calcium concentration in the cytosol. The high reproducibility of this assay allows monitoring HTR2A receptor activation process in living cells.
Cell line used: HiTSeeker 5HTR2A Cell Line
Readout: Calcium Flux
EC50 Agonist: 1.40 x 10-8 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry