Intoxication of neurons with beta-amyloid (1-42) oligomers is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta oligomers. Aβ1-42 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective potential of a molecule.
In our assay, we incubate rat primary neurons with test compounds in parallel with Aβ1-42 peptide. After 24h incubation, we analyze different parameters related to all stages of the cell death process. Some of the endpoints we analyze are cell viability, LDH Release, Neurite Outgrowth or Caspase activation.
Cell line used: Rat Primary Neurons
Readout: Neurite Outgrowth, LDH Release, Caspase Activation, Cell Viability
Damage Induction: Aβ1-42 Oligomers
Type of Assay: Cell-based / Neuroprotection