Amyloid-beta peptide Oligomerization Assay is a functional cell-based assay to identify inhibitors of amyloid peptide oligomerization process. We incubate rat primary neurons, human Microglia or SH-SY5Y cells with test compounds in parallel with Aβ1-42 peptide during 24h. After that, we measure Aβ1-42 oligomerization process analysing the number of thiazine staining oligomers related to cell number
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Cell line used: Rat primary Neurons, Immortalized Human Microglia or SH-SY5Y cell line
Readout: Stained oligomers quantification
Agonist: Aβ1-42 peptide (25 μM)
Assay Type: Cell-based
Detection Method: Image Analysis
Positive Compound: Galantamine (3 μM)
Assay: Alzheimer, Alzheimer's Disease, CNS, Modelos de enfermedad, Fenotípica, Área terapéutica
Area: Alzheimer's Disease, CNS