Androgen Receptor Translocation Assay

Androgen Receptor Translocation Assay from Innoprot analyzes stimuli for their ability to modulate receptor nuclear translocation process. To perform this assay, we quantify the fluorescence distribution inside the cells with a High Content Imaging system.

Human androgen receptor (hAR) is a ligand-dependent transcription factor responsible for the development of the male phenotype. The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. Androgenic hormones, such as testosterone or dihydrotestosterone binds the Androgen Receptor activating it.

This reproducible assay allows monitoring androgen receptor nuclear translocation process in High Content Analysis and fluorescence microscope applications.

Cell line used: tGFP-hAR HEK293 Cell Line

Readout: Nuclear Translocation

Agonist: Testosterone

EC50 Agonist: 2 × 10-10 M

Type of Assay: Cell-based / Functional

Detection Method: Image Analysis

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