Our Astrocytosis assay enables the study of fibrosis-related parameters on TGF-β1 induced Human primary astrocytes. Astrocytosis assay from Innoprot allows the evaluation of therapeutic compounds in this disease area by measuring several biomarkers; including Fibronectin, ASMA and also Collagen via high content analysis.
Astrocytosis is a common neurocellular manifestation of brain pathology in individuals with a variety of diseases. Expression of TGF-β1 occurs rapidly after CNS injury and activates transcription factors which increases fibronectin, collagen and also actin expression. Disease-modifying therapy that prevents or slows astrocytosis trans-differenciation is still lacking, therefore there is an urgent need to develop new therapies of neuroprotection against TGF-β1 astrocyte trans-differenciation.
Cell line used: Human Astrocytes
Readout: Fibronectin, ASMA & collagen expression
Agonist: TGF-β
Type of assay: Cell-based
Fibrosis Induction: TGF-β
Reference inhibitor: SB525334