Apoptosis, or programmed cell death, is a normal component of the development and health of multicellular organisms. Caspase 3-7 Activity Assay from Innoprot quantifies the apoptotic level in living cells. Apoptosis evaluation study is a cellular assay to evaluate the activation level of the caspase 3 protein which is a key role component during apoptosis activation. To evaluate the Caspase 3/7 activity, we use a fluorescent caspase 3/7 detection dye. We incubate the test-compounds with the cells and then we add the fluorescent dye. We finally measure fluorescent intensity in an automated fluorescent image platform (Cell Insight CX7 HCS Platform). In this assay, we finally compare the fluorescent signal of test-compounds with the control samples.
Cell line used: You can make your selection
Readout: Caspase 3-7 Activation fluorescent dye
Assay: Análisis de Daño Celular, Fenotípica, Toxicidad
Area: Cell Damage Assays