D1 Dopamine Receptor Assay from Innoprot allows the measurement of DRD1 receptor activation by cAMP increase in cell cytosol. We assay test-compounds analyzing the G-Protein signalling pathway by Gs involving DRD1 receptor activation. When an agonist binds to DRD1, it activates the Gs protein which; in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of fluorescence intensity of cAMP Nomad biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Cell line used: DRD1 Nomad Cell Line
Readout: cAMP Flux (Gs activation)
EC50 Agonist: 1.22 x 10-7 M for cAMP Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry