EP4 Prostanoid Receptor Assay from Innoprot measures PTGER4 receptor activation using HTRF technology. PTGER4 Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and also downstream L-type calcium channel interaction.
In this assay, we measure the receptor activation/inhibition by cAMP accumulation using HTRF technology. The high reproducibility of this assay allows monitoring EP4 Prostanoid Receptor Assay activation process in living cells.
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Cell line used: HiTSeeker PTGER4 Cell Line
Readout: cAMP Flux (HTRF)
Agonist: Prostaglandin E2
EC50 Agonist: 6.83 x 10-9 M for cAMP Flux
Type of Assay: Cell-based / Functional
Detection Method: HTRF