EP4 Prostanoid Receptor Assay

EP4 Prostanoid Receptor Assay from Innoprot measures PTGER4 receptor activation using HTRF technology. PTGER4 Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and also downstream L-type calcium channel interaction.

In this assay, we measure the receptor activation/inhibition by cAMP accumulation using HTRF technology. The high reproducibility of this assay allows monitoring EP4 Prostanoid Receptor Assay activation process in living cells.

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Cell line used: HiTSeeker PTGER4 Cell Line

Readout: cAMP Flux (HTRF)

Agonist: Prostaglandin E2

EC50 Agonist: 6.83 x 10-9 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: HTRF

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