FPR2/ALX Formylpeptide Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate FPR2/ALX receptor activation and the following redistribution process inside the cells. When a ligand binds to the FPR2/ALX, it activates a G protein, which internalizes in big and high intensity vesicles. This assay has been validated measuring Fluorescent FPR2/ALX redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring FPR2/ALX Formylpeptide Receptor redistribution process in High Throughput Screening.
Cell line used: Green Fluorescent FPR2/ALX Formylpeptide Receptor Cell Line
Readout: Fluorescent Receptor Internalization
EC50 Agonist: 1.50 x 10-5 ug/ml for receptor internalization
Assay type: Cell-based
Detection Method: Fluorescent Redistribution