Follicle stimulating hormone (FSH) is one of the gonadotrophic hormones, as well as luteinising hormone. It regulates the development, growth, pubertal maturation, and reproductive processes of the body. FSH and luteinizing hormone (LH) work together in the reproductive system. FSH is used commonly in infertility therapy, mainly for ovarian hyperstimulation as part of IVF. In some cases, reversal of anovulation treatments requires FSH as well. FSH Functional Assay from Innoprot allows the measurement of the activity and potency of recombinant human FSH (rhFSH).
To analyze the activity of rhFSH, we use a stable cell line stably expressing Follicle stimulating hormone receptor (FSHR). This cell line also expresses the cAMP-Nomad biosensor, which increases its fluorescence intensity upon FSHR activation. When rhFSH binds to the FSHR, it activates a G protein, which in turn triggers a cellular response mediated by second messengers (cAMP). This cAMP-Nomad FSHR cell line measures cAMP increase in the cytosol in a dose-response way in presence of rhFSH.
Cell line used: cAMP-Nomad FSHR Cell Line
Readout: cAMP increase
EC50 Agonist: 0.02 IU/ml
Type of assay: Cell-based / Functional
Detection Method: Fluorimetry