Glucocorticoid Receptor Translocation Assay from Innoprot analyzes stimuli for their ability to modulate receptor nuclear translocation process. To perform this assay, we quantify the fluorescence distribution inside the cells with a High Content Imaging system.
Glucocorticoid receptors (GRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid, progesterone and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. Glucocorticoids exert anti-inflammatory effects principally by repressing expression of the transcription factors AP-1 and NF-κB, and repression of pro-inflammatory genes. GR signaling facilitates an interface between the endocrine stress response and the immune system that is essential for restoring immune homeostasis following a response to stress. Glucocorticoid hormones, such as dexamethasone, cortisol or deoxycorticosterone binds the Glucocorticoid Receptor activating it.
This reproducible assay allows monitoring glucocorticoid receptor nuclear translocation process in High Content Analysis and fluorescence microscope applications.
Cell line used: tGFP-hGR HEK293 Cell Line
Readout: Nuclear Translocation
EC50 Agonist: 6 × 10-10 M
Type of Assay: Cell-based/Functional
Detection Method: Image Analysis