Histamine H1 Receptor Assay

Histamine H1 Receptor Assay from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay.

Histamine 1 Receptor stimulation leads to the synthesis and release of several neurochemicals and neuromodulators; such as prostacyclin, platelet–activating factor, and nitric oxide (NO). In addition, stimulation of the H1 receptor causes changes in vascular permeability, particularly the postcapillary venule as a result of endothelial cell contraction. Antihistamines, which act on this receptor, are anti-allergy drugs.

Receptor Information: Histamine H1 Receptor

Cell line used: HRH1 Nomad Cell Line

Readout: Calcium Flux & β-arrestin recruitment

Agonist: Histamine

EC50 Agonist: 6.93 x 10-8 M for Calcium Assay & 3.86 x 10-8 for β-arrestin recruitment

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

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