Kappa Opioid Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate Kappa Opioid receptor (OPRK1) activation and the following redistribution process inside the cells. When a ligand binds to the OPRK1, it activates a G protein, which internalizes in big and high intensity vesicles.
This assay has been validated measuring Fluorescent OPRK1 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring Kappa Opioid Receptor redistribution process in High Throughput Screening.
Receptor Information: Kappa Opioid Receptor
Cell line used: Green Fluorescent Kappa Opioid Receptor Cell Line
Readout: Fluorescent Receptor Internalization
Agonist: U-50488
EC50 Agonist: 3.42 x 10-10 M for receptor internalization
Assay type: Cell-based
Detection Method: Fluorescent Redistribution
Assay: Opioid Receptors