Kappa Opioid Receptor Internalization Assay

Kappa Opioid Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate Kappa Opioid receptor (OPRK1) activation and the following redistribution process inside the cells. When a ligand binds to the OPRK1, it activates a G protein, which internalizes in big and high intensity vesicles.

This assay has been validated measuring Fluorescent OPRK1 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring Kappa Opioid Receptor redistribution process in High Throughput Screening.

Receptor Information: Kappa Opioid Receptor

Cell line used: Green Fluorescent Kappa Opioid Receptor Cell Line

Readout: Fluorescent Receptor Internalization

Agonist: U-50488

EC50 Agonist: 3.42 x 10-10 M for receptor internalization

Assay type: Cell-based

Detection Method: Fluorescent Redistribution

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