M4 Muscarinic Acetylcholine Receptor Assay (CHRM4) from Innoprot measures CHRM4 receptor activation using Nomad Biosensors technology. CHRM4 signaling occurs via adenylate cyclase inhibition through trimeric Gi/o proteins, reducing cAMP via PKA activation.
In this assay, we measure the receptor activation/inhibition by PKA activation using Nomad Biosensors technology. The high reproducibility of this assay allows monitoring M4 Muscarinic Acetylcholine Receptor activation process in living cells.
Receptor Information: M4 Muscarinic Acetylcholine Receptor
Cell line used: CHRM4 Nomad Cell Line
Readout: cAMP Flux (Gi/o activation)
Agonist: Oxotremorine
EC50 Agonist: 4.72 x 10-8 M for Gi/Go Activation
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry
Assay: Muscarinic Receptors