The oxidative stress is an imbalance between the production of reactive oxygen strains (ROS); and the capacity of the cell to remove them. The excess of these strains finally produce peroxides and free radicals that damage cell components and compromise the cell functions. Oxidative Stress Assay from Innoprot uses a specific probe (CM-H2DCFDA) to evaluate the redox level; which in contact with the reactive oxygen strains produces a specific fluorescence. Using this dye, It is possible to evaluate the redox status in living cells. We incubate the cells with test-compounds measuring the fluorescent signal respect to control with the Cell Insight CX7 HCS platform.
Cell line used: You can make your selection
Readout: General Oxidative Stress Indicator - CM-H2DCFDA
Assay: Análisis de Daño Celular, Fenotípica, Toxicidad
Area: Cell Damage Assays