PAC1 Receptor Assay from Innoprot measures PAC1 receptor activation using HTRF technology. PAC1 Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction.
In this assay, we measure the receptor activation/inhibition by cAMP accumulation using HTRF technology. The high reproducibility of this assay allows monitoring PAC1 Receptor activation process in living cells.
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Cell line used: HiTSeeker PAC1R Cell Line
Readout: cAMP Flux (HTRF)
EC50 Agonist: 5.13 x 10-10 M for cAMP Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry