Renal Fibrosis Assay from Innoprot enables the study of fibrosis-related parameters on TGF-β induced Renal Tubular Epithelial Cells.
Human Renal Tubular Epithelial Cells stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in αSMA levels; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is inhibited by treatment with the ALK-5 inhibitor, SB525334, showing full inhibition of αSMA regardless of the presenceof TGF-β1. Two different ways to measure changes are performed, actin stained area and fiber length.
Cell line used: Human Renal Tubular Epithelial Cells
Readout: ASMA expression & distribution
Agonist: TGF-β