S1P1 Lysophospholipid Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate S1P1 receptor activation and the following redistribution process inside the cells. When a ligand binds to the S1P1R, it activates a G protein, which internalizes in big and high intensity vesicles.
This assay has been validated measuring Fluorescent S1P1R redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring S1P1 Lysophospholipid Receptor redistribution process in High Throughput Screening.
Cell line used: Green Fluorescent S1P1 Lysophospholipid Receptor Cell Line
Readout: Fluorescent Receptor Internalization
EC50 Agonist: 6.70 x 10-7 M for receptor internalization
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry