S1P3 Lysophospholipid Receptor Assay (S1P3R) from Innoprot provides the analysis of both signalling pathways involving S1P3R activation; G Protein by the analysis of Gi/Go activation and also β-arrestin recruitment using Nomad multiplex technology. When an agonist binds to S1P3R, it activates Gi/Go, which in turn, triggers a cellular response involving PKA activation. In this assay we measure Gi/Go activation in combination with β-arrestin recruitment.
The high reproducibility of this assay allows monitoring S1P3 Lysophospholipid Receptor activation process in High Throughput Screening.
Receptor Information: S1P3 Lysophospholipid Receptor Receptor
Cell line used: S1P3R Nomad Cell Line
Readout: Gi/Go Activation & β-arrestin recruitment
EC50 Agonist: 7.14 x 10-7 M for Gi/Go Assay & 9.30 x 10-7 for β-arrestin recruitment
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry