VPAC2 Receptor Assay (VIPR2) from Innoprot measures VIPR2 activation using Nomad Biosensors technology. VPAC2 Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction.
In this assay, we measure the receptor activation/inhibition by cAMP accumulation using cAMP-Nomad Biosensor. The high reproducibility of this assay allows monitoring VPAC2 Receptor activation process in living cells.
VPAC2 Receptor is present throughout the body and is also present in a variety of human neoplastic tissues. VPAC2 may play a role in schizophrenia, but also the abnormal expression of VIPR2 messenger RNA in gallbladder tissue may play a role in the formation of gall stones and polyps.
Cell line used: VIPR2 Nomad Cell Line
Readout: cAMP Flux (Gs activation)
Agonist: PACAP-38
EC50 Agonist: 1.28 x 10-8 M for cAMP Flux
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry