CB1 Cannabinoid Receptor Assay

CB1 Cannabinoid Receptor Assay

Human CB1 cannabinoid receptor assay (CNR1) from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G Protein by the analysis of Gi/Go activation and β-arrestin recruitment using Nomad multiplex technology. When an agonist binds to CNR1, it activates Gi/Go, which in turn, triggers a cellular response involving PKA activation. In this assay…

Glucocorticoid Receptor Translocation Assay

Glucocorticoid Receptor Translocation Assay

Glucocorticoid Receptor Translocation Assay from Innoprot analyzes stimuli for their ability to modulate receptor nuclear translocation process. To perform this assay, we quantify the fluorescence distribution inside the cells with a High Content Imaging system. Glucocorticoid receptors (GRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid, progesterone and androgen receptors. They…

FSH Functional Assay

FSH Functional Assay

Follicle stimulating hormone (FSH) is one of the gonadotrophic hormones, as well as luteinising hormone. It regulates the development, growth, pubertal maturation, and reproductive processes of the body. FSH and luteinizing hormone (LH) work together in the reproductive system. FSH is used commonly in infertility therapy, mainly for ovarian hyperstimulation as part of IVF. In…

LHCGR Functional Assay

LHCGR Functional Assay

The luteinizing hormone/choriogonadotropin receptor (LHCGR), also lutropin/choriogonadotropin receptor (LCGR) or luteinizing hormone receptor (LHR) is a transmembrane receptor found predominantly in the ovary and testis, but also many extragonadal organs such as the uterus and breasts. The receptor interacts with both luteinizing hormone (LH) and chorionic gonadotropins (such as hCG in humans). Its activation is…

Gamma secretase activity assay

Gamma secretase activity assay

Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.

tau Phosphorylation Assay

tau Phosphorylation Assay

tau phosphorylation assay from Innoprot allows the identification of tau kinase and phosphatase inhibitors and modulators that affect the behaviour or location of tau protein. Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing. They accumulate in neurofibrillary tangles in Alzheimer’s disease (AD) brain. There is significant evidence that…