Alzheimer’s Disease (AD) comprises the accumulation of brain depositions of the beta amyloid (βA). The βA is the amyloid precursor protein (APP) digestion product, released from the cell after secretases proteolysis.This is the basis of the APP Processing Assay offered by Innoprot. Innoprot has developed a novel fluorescence-based assay for screening of inhibitors of the…
Parkin mitochondrial recruitment assay from Innoprot is based of localization changes of two fluorescent proteins; the Parkin fluorescent fusion polypeptide that changes its localization from the cytosol to the outer mitochondrial membrane (OMM); and the other fluorescent fusion polypeptide (MTS-tGFP), which binds directly to OMM, allowing the mitochondrial integrity analysis. Several compounds promote Parkin recruitment,…
Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative disease of the motor neuron system. The TDP-43 pathology seems to be a dominant type of pathology across sporadic ALS types. Our TDP-43 Stress Granules Assay allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model also permits to monitor…
FUS/TLS Stress Granules Assay from Innoprot requires a stable cell line expressing fluorescent FUS/TLS. During the assay, we incubate cells with test compounds and Sodium Arsenite to induce the FUS/TLS aggregation. Finally, we quantify fluorescent FUS/TLS nuclear globs using the BD Pathway HCS Reader and Attovision Compartimentalization Software. Amyotrophic lateral sclerosis (ALS) is one of…
A brief exposure to glutamate in vitro causes neuronal death, which makes glutamate a useful tool to evaluate neuroprotective activities. Innoprot excitotoxicity in vitro assay offers the advantage of a rapid analysis of the neuroprotective potential of a molecule. In our assay, we incubate rat primary neurons with test compounds 24 hours before L-glutamate insult.…
Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.
tau phosphorylation assay from Innoprot allows the identification of tau kinase and phosphatase inhibitors and modulators that affect the behaviour or location of tau protein. Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing. They accumulate in neurofibrillary tangles in Alzheimer’s disease (AD) brain. There is significant evidence that…