In vitro Cell Invasion Assay

In vitro Cell Invasion Assay

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix. In vitro cell invasion assay from Innoprot is a Boyden Chamber assay. Boyden Chambers consist of a cell culture insert nested in the…

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro assay is based on the exposure of rat primary neurons under Oxygen-Glucose Deprivation conditions. Ischemia-like conditions are induced by replacing the normal O2/CO2 equilibrated medium with 94,7% N2 /5% CO2/0,3% O2 medium in a hypoxic chamber. We also use a culture medium without glucose to expose the cells to real OGD…

Amyloid-beta peptide Oligomerization Assay

Amyloid-beta peptide Oligomerization Assay

Amyloid-beta peptide Oligomerization Assay is a functional cell-based assay to identify inhibitors of amyloid peptide oligomerization process. We incubate rat primary neurons, human Microglia or SH-SY5Y cells with test compounds in parallel with Aβ1-42 peptide during 24h. After that, we measure Aβ1-42 oligomerization process analysing the number of thiazine staining oligomers related to cell number .

APP Processing Assay

APP Processing Assay

Alzheimer’s Disease (AD) comprises the accumulation of brain depositions of the beta amyloid (βA). The βA is the amyloid precursor protein (APP) digestion product, released from the cell after secretases proteolysis.This is the basis of the APP Processing Assay offered by Innoprot. Innoprot has developed a novel fluorescence-based assay for screening of inhibitors of the…

Parkin Mitochondrial Recruitment Assay

Parkin Mitochondrial Recruitment Assay

Parkin mitochondrial recruitment assay from Innoprot is based of localization changes of two fluorescent proteins; the Parkin fluorescent fusion polypeptide that changes its localization from the cytosol to the outer mitochondrial membrane (OMM); and the other fluorescent fusion polypeptide (MTS-tGFP), which binds directly to OMM, allowing the mitochondrial integrity analysis. Several compounds promote Parkin recruitment,…

TDP-43 Stress Granules Assay

TDP-43 Stress Granules Assay

Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative disease of the motor neuron system. The TDP-43 pathology seems to be a dominant type of pathology across sporadic ALS types. Our TDP-43 Stress Granules Assay allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model also permits to monitor…

FUS/TLS Stress Granules Assay

FUS/TLS Stress Granules Assay

FUS/TLS Stress Granules Assay from Innoprot requires a stable cell line expressing fluorescent FUS/TLS. During the assay, we incubate cells with test compounds and Sodium Arsenite to induce the FUS/TLS aggregation. Finally, we quantify fluorescent FUS/TLS nuclear globs using the BD Pathway HCS Reader and Attovision Compartimentalization Software. Amyotrophic lateral sclerosis (ALS) is one of…

Excitotoxicity in vitro assay

Excitotoxicity in vitro assay

A brief exposure to glutamate in vitro causes neuronal death, which makes glutamate a useful tool to evaluate neuroprotective activities. Innoprot excitotoxicity in vitro assay offers the advantage of a rapid analysis of the neuroprotective potential of a molecule. In our assay, we incubate rat primary neurons with test compounds 24 hours before L-glutamate insult.…

Neurite Outgrowth Assay

Neurite Outgrowth Assay

Neurite outgrowth is a key process during neurogenesis. During development, neurons extend numerous processes that differentiate into dendrites and axons. These processes, also termed neurites, are critical for communication between neurons. The initiation of neurite protrusion and subsequent elongation involves complex intracellular signaling. Neurites are particularly interesting in relation to neuropathological disorders, neuronal injury/regeneration, and…