M1 Muscarinic Acetylcholine Receptor Assay (CHRM1) from Innoprot allows to assay compounds, or analyze their capability to modulate CHRM1. When a ligand binds to the CHRM1, it activates a G protein; which in turn, triggers a cellular response mediated by calcium. The binding of an agonist to CHRM1 induces mobilization of intracellular calcium and also activation of PKC via the PLC–IP3 and DAG pathways, respectively.
M1 Muscarinic Acetylcholine Receptor Assay comprises the measurement of calcium concentration in the cytosol. The high reproducibility of this assay allows monitoring CHRM1 receptor activation process in living cells.
Receptor Information: M1 Muscarinic Acetylcholine Receptor