cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD ADRB2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human B2 adrenoceptor with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD B2 adrenoceptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by cAMP involving receptor activation. When an agonist binds to ADRB2 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent beta-2 adrenoreceptor Cell Line contains CHO-K1 cells stably expressing ADRB2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent beta-2 adrenoreceptor Cell Line allows to assay compounds, or analyze their capability to modulate ADRB2 activation and the following redistribution process inside the cells. When a ligand binds to the ADRB2, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent ADRB2 redistribution by image analysis as an increment of fluorescent intensity of these vesicles. The high reproducibility of this assay allows monitoring ADRB2 receptor redistribution process in High Throughput Screening.
HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker beta-2 adrenergic receptor Cell Line contains HEK293 cells stably expressing ADRB2 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.
Innoprot HiTSeeker ADRB2 Cell Line allows to assay compounds, or analyze their capability to modulate ADRB2. When a ligand binds to the ADRB2, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker ADRB2 cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with Isoproterenol. The high reproducibility of this assay allows monitoring ADRB2 receptor activation process in High Throughput Screening.
