Mostrando todos los resultados (3)

CCK2 Cholecystokinin Receptor Cell LineCCK2 Cholecystokinin Receptor Cell Line

MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human CCK2 Cholecystokinin Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Lineβ-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line

β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line

9.995,00

β-ArNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and β-arrestin Nomad Biosensor to GPCR β-arrestin modulation. Each vial of β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human CCK2 Cholecystokinin Receptor with no tag
  • β-arrestin Nomad Biosensor (Green Fluorescence)

β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing β-arrestin recruitment. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. Due to the presence of ß-Arrestin Nomad Biosensor, this recruitment leads to an increase of green fluorescence intensity. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

HiTSeeker Cholecystokinin B receptor Cell LineHiTSeeker Vasopressin Receptor 1A Cell Line

HiTSeeker Cholecystokinin B receptor Cell Line

5.250,00

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker Cholecystokinin A receptor Cell Line contains U2OS cells stably expressing CCKBR with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker Cholecystokinin B receptor Cell Line allows to assay compounds, or analyze their capability to modulate CCKBR. When a ligand binds to the CCKBR, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker CCKBR cell line has been validated measuring calcium concentration changes in the cytosol upon activation with CCK Octapeptide sulfated. The high reproducibility of this assay allows monitoring CCKBR receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial