cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD D5 Dopamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human D5 Dopamine Receptor (DRD5) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD DRD5 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD5, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent D5 Dopamine Receptor Cell Line contains SH-SY5Y cells stably expressing DRD5 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent D5 Dopamine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate DRD5 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the DRD5 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent D5 Dopamine Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring DRD5 Receptor redistribution process in High Throughput Screening.
HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker D5 Dopamine Receptor Cell Line contains HEK293 cells stably expressing DRD5 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.
Innoprot HiTSeeker DRD5 Cell Line allows to assay compounds, or analyze their capability to modulate DRD5. When a ligand binds to the DRD5, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker DRD5 cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with Dopamine. The high reproducibility of this assay allows monitoring DRD5 receptor activation process in High Throughput Screening.
Green Fluorescent D5 Dopamine Receptor Cell Line 
