cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD GLP-2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human GLP-2 Receptor (GLP2R) with no tag
cAMP Nomad Biosensor (Red Fluorescence)
cAMPNOMAD GLP-2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GLP-2, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent GLP-2 Receptor Cell Line contains U2OS cells stably expressing GLP2R tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent GLP-2 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate GLP2R activation and the following redistribution process inside the cells. When a ligand binds to the GLP-2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent GLP2R redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring GLP-2 Receptor redistribution process in High Throughput Screening.
