Mostrando todos los resultados (2)

NTS1 Neurotensin Receptor Cell Line

MPXNOMAD NTS1 Neurotensin Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD NTS1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human NTS1 Neurotensin Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD NTS1 Neurotensin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to NTS1R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

NTSR1 Neurotensin Receptor Cell LineCalcium Assay

HiTSeeker NTSR1 Neurotensin Receptor Cell Line

5.250,00

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker NTSR1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing NTSR1 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker NTSR1 Neurotensin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate Neurotensin 1 receptor. When a ligand binds to the NTSR1, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker NTSR1 Receptor cell line has been validated measuring calcium concentration changes in the cytosol upon activation with Neurotensin. The high reproducibility of this assay allows monitoring NTSR1 activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial