Mostrando todos los resultados (2)

NOMAD NK2 Tachykinin Receptor Cell LineNK2R

MPXNOMAD NK2 Tachykinin Receptor Cell Line

19.950,00

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor both G-protein pathways. Each vial of MPXNOMAD NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human NK2 Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • cAMP Nomad Biosensor (Red Fluorescence)

MPXNOMAD NK2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G proteins by Ca2+ & cAMP flux, hence allowing biased activity studies. When an agonist binds to NK2 Receptor two G proteins are activated which, in turn, triggers a cellular response mediated by calcium and cAMP. This leads to an increase of fluorescence intensity of both cAMP and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

NK2 Tachykinin Receptor Cell LineCalcium Assay

HiTSeeker NK2 Tachykinin Receptor Cell Line

5.250,00

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing NK2 Receptor with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker NK2 Tachykinin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate NK2 receptor. When a ligand binds to the NK2R, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker NK2 Receptor cell line has been validated measuring calcium concentration changes in the cytosol upon activation with Substance P. The high reproducibility of this assay allows monitoring NK2 Receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial