The cervix is the lower portion of the uterus. It functions to promote fertility and protect the female reproductive tract and also fetus from potential pathogens. In addition, the cervix plays a critical role in childbirth by both; effacing and dilating to allow the baby to pass out of the uterus. Human Cervical epithelial cells (CerEpiC), which line the cervix, maintain structural integrity and limit the passage of molecular and cellular substances into the cervix using intercellular junctions. Patients infected with HPV causes inflammation of the epithelial cell layer which can lead to the development of cervical carcinoma. Primary human CerEpiC (HCerEpiC) are a useful in vitro model for studying the pathophysiology of cervical polyps, HPV, and cervical cancer. Human Cervical Epithelial Cells are also useful tools to develop disease models for High Throughput and High Content Screening.