_cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of _cAMPNOMAD Adenosine A1 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

• human Adenosine A1 receptor (ADORA1) with no tag
• cAMP Nomad Biosensor (Red Fluorescence)

_cAMPNOMAD ADORA1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA1, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial