Fibroblast Medium-2 (FM) is a complete medium designed for optimal growth of normal human cardiac fibroblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human fibroblasts in vitro.
Fibroblast Medium Kit is a complete medium designed for optimal growth of normal primary fibroblasts in vitro. Each Fibroblast Medium Kit consists of the next components:
- 500 ml of Fibroblast basal medium
- 25 ml of fetal bovine serum
- 5 ml of Fibroblast Growth supplement-2
- 5 ml of penicillin/streptomycin solution.