Green Fluorescent A375 Cell Line

Reference: P20122

Green Fluorescent A375 Cell Line

Green fluorescent A375 cell line (aka LINTERNA A375) has been developed through stable transfection of A375 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:

LINTERNA A375 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent A375 Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.

950,00

Green Fluorescent A375 Cell Line are suitable for in vitro and in vivo experimentation.

About A375 Cell line:

A375 melanoma cell line derives from the skin of a 54 year-old female patient with malignant melanoma. These tumorigenic cells exhibit an epithelial cellular morphology, and are useful as a stable transfection host. A375 can be useful for molecular and cell biology research, particularly related to skin cancer. IL1-alpha or IL1-beta inhibit growth of A375 cells, which cause growth arrest in phase G0/G1 of the cell cycle. Accordingly, A375 cells are useful in research on cytokines for bioassays of these factors. This cell line also produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P20122
  • Size/Quantity: 3x106 cells / vial
  • Resistance Gene: G418
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice