Green Fluorescent A549 Cell Line

Reference: P20118

Green Fluorescent A549 Cell Line

Green Fluorescent A549 Cell Line (aka LINTERNA A549) has been developed through stable transfection of A549 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:

LINTERNA A549 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent A549 Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.


Green Fluorescent A549 Cell Line are suitable for both, in vitro and in vivo experimentation.

About A549 Cell line:

A549 cell line is an adenocarcinomic human alveolar basal epithelial cell line got through the removal and culturing of cancerous lung tissue in an explanted tumor. In nature, these cells are squamous and responsible for the diffusion of some substances; such as water and electrolytes, across the alveoli of lungs. A549 cells cultures in vitro grow as monolayer cells, adherent or attaching to the culture flask. Another characteristic of these cells is that they are able to synthesize lecithin and also contain high level of desaturated fatty acids. A549 cell line is useful as in vitro model for a type II pulmonary epithelial cell model for drug metabolism.

About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P20118
  • Size/Quantity: 3x106 cells / vial
  • Resistance Gene: G418
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice