Green Fluorescent Immortalized HDMEC

Reference: P10861-IM-tGFP

Green Fluorescent Immortalized HDMEC

Green Fluorescent Immortalized Human Dermal Microvascular Endothelial Cells (IM-HDMEC-tGFP) provided by Innoprot have been developed by immortalizing primary human dermal endothelial cells with SV40 Large T antigen and transfecting with tGFP.

Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.

Each vial of IM-HDMEC-tGFP contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HDMEC-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HDMEC-tGFP culture.

 

Size/Quantity: 1 million cells / vial

2.950,00

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About HDMEC:

Blood vessels of the skin contains both, cellular and non-cellular elements. The predominant cell type in all skin blood vessels is endothelial cells. Endothelial cells form the interface between intravascular and extravascular compartments. They serve as a selective barrier for the diffusion of cells and macromolecules between these two compartments. While endothelial cells lining blood vessels of the skin share features of endothelial cells elsewhere in the body, they also exhibit several skin specific characteristics. Human Dermal Microvascular Endothelial Cells provide an invaluable tool for understanding DMEC physiological and pathophysiological relevance in dermal function. Immortalized Human Dermal Microvascular Endothelial Cells also allow to stablish in vitro models for HIgh Throughput and High Content Screening.

 

About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

 

  • Reference: P10861-IM-tGFP
  • Size/Quantity: 1x106 cells / vial
  • Fluorescent Protein: turboGFP (excitation/emission max = 482/502 nm respectively)
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice