Green Fluorescent S1P1 Lysophospholipid Receptor Cell Line

Reference: P30226

Green Fluorescent S1P1 Lysophospholipid Receptor Cell Line

Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent S1P1 Lysophospholipid Receptor Cell Line contains HepG2 cells stably expressing S1P1R tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.

Innoprot Green Fluorescent S1P1 Lysophospholipid Receptor Cell Line allows to assay compounds, or analyze their capability to modulate S1P1R activation and the following redistribution process inside the cells. When a ligand binds to the S1P1 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent S1P1R redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring S1P1 Receptor redistribution process in High Throughput Screening.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

6.950,00

Categoría: Etiquetas:

Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. This receptor is a GPCR which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. S1P1 Receptor is target for a variety of disease indications and with positive results for the treatment of patients with relapsing multiple sclerosis. Green Fluorescent S1P1 Lysophospholipid Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as S1P.

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  • Reference: P30226
  • DNA Accession Number: NM_001400
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice