Green Fluorescent SH-SY5Y Cell Line are suitable for both, in vitro and in vivo experimentation.
About SH-SY5Y Cell line:
SH-SY5Y is a clonal subline of the neuroepithelioma cell line SK-N-SH derived from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma. They are epithelial- /neuronal-like cells growing as monolayer and in cell clusters. SH-SY5Y cells are dopamine beta hydroxylase active, acetylcholinergic, glutamatergic and also adenosinergic. The cells have very different growth phases and propagate via mitosis and differentiate by extending neurites to the surrounding area. Some treatments such as retinoic acid and BDNF can force the cells to dendrify and differentiate.
About turboGFP protein:
tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.