HiTSeeker Adenosine A2B Receptor Cell Line

Reference: P30192

HiTSeeker Adenosine A2B Receptor Cell Line

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker Adenosine A2B Receptor Cell Line contains HEK293 cells stably expressing ADORA2B with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ADORA2B Cell Line allows to assay compounds, or analyze their capability to modulate ADORA2B. When a ligand binds to the ADORA2B, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker ADORA2B cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with NECA. The high reproducibility of this assay allows monitoring ADORA2B receptor activation process in High Throughput Screening.


Size/Quantity: 2 vials containing 3×106 cells / vial


HiTSeeker Adenosine A2B Receptor Cell Line comprises HEK293 cells stably expressing ADORA2B.

ADORA2B gene encodes a protein which is one of receptor subtypes for adenosine, abundant in large intestine and bladder. A2B receptors plays an important role in mast cell activation, asthma, vasodilation, regulation of cell growth and intestinal function. The presence of a HIF-1alpha binding site in the promoter region of the A2b receptor gene implicates the receptor in hypoxia and angiogenesis. Targeting the A2b receptor may also be useful in combating autoimmune type I diabetes.

Adenosine A2B Receptor have multiple binding partners that modulate ADORA2B responses and functions; these include netrin-1, E3KARPP-EZRIN-PKA, SNARE, NF-B1/P105, and -actinin-1. Netrin-1, the neuronal guidance molecule, induced during hypoxia, reduces inflammation by activating ADORA2B, which inhibit neutrophils migration. SNARE protein interacting with ADORA2B, mostly that located inside the cell, recruits the receptor to the plasma membrane following agonist binding. After this interaction, a multiprotein complex with E3KARP (NHERF2) and ezrin stabilizes ADORA2B in the plasma membrane.

  • Reference: P30192
  • Qty: 3 million cells/vial
  • Products use: For research purposes only
  • Shipping conditions: Dry Ice