HiTSeeker CB1 Cannabinoid Receptor Cell Line

Reference: P30107

HiTSeeker CB1 Cannabinoid Receptor Cell Line

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing CB1 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker CB1 Cell Line allows to assay compounds, or analyze their capability to modulate CB1. When a ligand binds to the CB1, it activates a G protein, which in turn, triggers a cellular response mediated by a second messenger. HiTSeeker CB1 cell line has been validated performing an assay with [3H]CP55940 and increasing concentrations of no radioactive competitor. The high reproducibility of this assay allows monitoring CB1 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial


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The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two cannabinoid receptors play and important role in the cannabinoid-induced CNS effects, including alterations in mood and cognition. CB1 receptors appear in the CNS and are responsible for mediating the effects of cannabinoid binding in the brain. Depolarized neurons release endocannabinoids, which bind to CB1 receptors on pre-synaptic glutamatergic and GABAergic neurons, resulting in a respective decrease in either glutamate or GABA release. HiTSeeker CB1 Cannabinoid Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as CP55940.

  • Reference: P30107
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice