HiTSeeker ETA Endothelin Receptor Cell Line

Reference: P30151

HiTSeeker ETA Endothelin Receptor Cell Line

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker ETA Endothelin Receptor Cell Line contains U2OS cells stably expressing ETA Receptor with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker ETA Endothelin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate ETA receptor. When a ligand binds to the ETA receptor, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker ETA Receptor cell line has been validated measuring calcium concentration changes in the cytosol upon activation with Endothelin agonist. The high reproducibility of this assay allows monitoring ETA receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial


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The endothelins and their receptors refers as the endothelin (ET) axis and this axis plays roles in diverse tumours. ETA is a subtype for vasoconstriction. Endothelin A receptor (ETA) favours blood presure by increasing vasoconstriction and sodium retention after ET binding; this is why it is more abundant in smooth muscle cells of blood vessels. Receptors for endothelin appear in blood vessels and cells of the brain, choroid plexus and peripheral nerves. When applied directly to the brain of rats in picomolar quantities as an experimental model of stroke, endothelin-1 caused severe metabolic stimulation and seizures with substantial decreases in blood flow to the same brain regions, both effects mediated by calcium channels. HiTSeeker ETA Endothelin Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as Endothelin.

  • Reference: P30151
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice