Endothelial cells lining the microvasculature play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Intestinal microvascular endothelial cells also exhibit a strong immune response to LPS challenge. It also plays a critical regulatory role in gut inflammation. Pharmacological inhibition of NOS in activated intestinal microvascular endothelial cells resulted in a significant increase in leukocytes binding. Gene expression profile studies reveals that intestinal endothelial cells express biotinidase, which involves in biotin recycling. Human colonic microvascular endothelial cells allow performing systematic analyses of cytokine profiles with regard to mRNA expression and protein secretion.
About turbo602FP protein:
TurboFP602 is a red-shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. TurboFP602 possesses true-red fluorescence (with excitation/emission maxima at 574/602 nm, respectively), optimal for detection via most popular filter sets, and is also easily distinguished from background signals. This red frluorescent protein exhibits fast maturation and high pH stability.
TurboFP602 is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.