Red Fluorescent Human Dermal Fibroblasts

Reference: P20204

Red Fluorescent Human Dermal Fibroblasts

Red Fluorescent Human Dermal Fibroblasts (Red TTFLUOR HDF) provided by Innoprot are isolated from human skin by explants (individual donors) and transfected with turboFP602. Red TTFLUOR HDF are cryopreserved at passage one and delivered frozen. Each vial of Red Fluorescent Human Dermal Fibroblasts contains more than 500.000 viable cells. They are guaranteed to further expand for 10 population doublings following the instructions provided in the technical sheet.

Innoprot also offers optimized medium and reagents for the growth of Red TTFLUOR Human Dermal Fibroblasts which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro HDF culture.

 

Size/Quantity: 500.000 Cells / vial

445,00

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About Human Dermal Fibroblasts:

Cultures of human dermal fibroblasts are very useful for a wide range of cellular and molecular studies. This is mainly because fibroblasts are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, human dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.

 

About turbo602FP protein:

TurboFP602 is a red-shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. TurboFP602 possesses true-red fluorescence (with excitation/emission maxima at 574/602 nm, respectively), optimal for detection via most popular filter sets, and is also easily distinguished from background signals. This red frluorescent protein exhibits fast maturation and high pH stability.

TurboFP602 is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity

  • Reference: P20204
  • Size/Quantity: 5x105 cells / vial
  • Fluorescent Protein: turboFP602 (excitation/emission max = 574/602 nm respectively)
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice

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