_cAMPNOMAD CALCRL Cell Line

_cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of _cAMPNOMAD CALCRL Receptor Cell Line contains U2OS cells stably expressing the following constructs:

• Human calcitonin receptor-like receptor (CALCRL) with no tag
• cAMP Nomad Biosensor (Red Fluorescence)

_cAMPNOMAD CALCRL Receptor Cell Line enables the assessment of compounds by analyzing the G-protein signalling pathway mediated by Gs proteins, following receptor activation. When an agonist binds to the receptor, a G protein is activated, triggering a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. The activity can be easily quantified in living cells in a dose-response manner, with fluorescence intensity detected and analyzed using a microplate reader, and images captured with an image analysis system.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial